Macrocyst Genetics in Polysphondylium pallidum, a Cellular Slime Mould

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Ultrastructural changes during formation and germination of microcysts in Polysphondylium pallidum, a cellular slime mould.

Ultrastructural changes during encystation of Polysphondylium palltdum, a cellular slime mould, include an increase in fibnllar material in the cytoplasmic matrix, the formation of cytoplasms microprojections at the cell periphery and the occurrence of tiny vesicles and some larger vacuoles near the cell periphery. The cyst wall appears first as a fluffy, loose network of fibrils. In the mature...

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Spots and stripes: the patterning spectrum in the cellular slime mould Polysphondylium pallidum

Whorls of the cellular slime mould Polysphondylium pallidum originate as spherical cell masses that during normal morphogenesis produce tips only at equidistant positions around their equator. We have observed a series of new patterns in whorls that differ from normal whorls only in that they are larger or more elongated. Among the novel patterns found were arrays of tips distributed fairly reg...

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Techniques and Marker Genes for Use in Macrocyst Genetics with POLYSPHONDYLIUM PALLIDUM.

Previous work has shown that genetic exchange occurs in the macrocyst of Polysphondylium pallidum, as in species of Dictyostelium. These studies are extended here. Mutants resistant to six different poisons have been isolated for use as genetic markers. A replica-plating technique has been engineered whereby 14 progeny clones growing on a master plate may be simultaneously transferred to test p...

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Alpha-mannosidase and microcyst differentiation in the cellular slime mold Polysphondylium pallidum.

The intracellular and extracellular pattern of alpha-mannosidase (EC 3.2.1.24) activity was studied during microcyst differentiation in the cellular slime mold, Polysphondylium pallidum. The evidence suggests that microcyst differentiation requires continuous protein synthesis. alpha-Mannosidase activity is present in amoebae and increases with differentiation, and the data indicate that this i...

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Spatial patterns in the fruiting bodies of the cellular slime mold Polysphondylium pallidum.

During morphogenesis in the slime mold Polysphondylium pallidum cell masses are periodically pinched off from the base of the developing sorogen. These masses round up and differentiate into secondary sorogens, which become radially ordered arrays of secondary fruiting bodies called whorls. Here we describe the morphogenesis of P. pallidum and characterize the spacing of whorls along the centra...

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ژورنال

عنوان ژورنال: Journal of General Microbiology

سال: 1975

ISSN: 0022-1287

DOI: 10.1099/00221287-89-2-310